McCoy’s5A 培养基
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- 产品描述
- COA
- 用户指南
- 配方
- 产品参数
|
货号 |
B1136-060 |
|
包含 |
L-谷氨酰胺，高糖，细菌蛋白胨，酚红 |
|
不含 |
丙酮酸钠，HEPES |
|
外观 |
清澈的红色液体 |
|
规格 |
500ML |
|
单价(¥） |
100 |
|
Triple 0.1µm sterile-filtered |
|
|
储存条件 |
2°C to 8°C (避光保存) |
|
运输条件 |
室温 |
|
保质期 |
自生产日期起12个月 |
McCoy's5A (modified)是一种通用培养基，可支持多种类型的原代细胞、已建立的细胞系和活检组织的外植体的繁殖。该培养基将支持来自正常骨髓、皮肤、脾脏、肾脏、肺、大鼠胚胎和其他组织的原始哺乳动物细胞的生长。
使用McCoy的5A(改良)培养基，Dr.Thomas McCoy最初将McCoy's 5A培养基配方为基础培养基5A的修饰。与其他培养基不同，McCoy's 5A含有还原剂谷胱甘肽、细菌-蛋白胨和高水平的葡萄糖。该产品还包括Hsu博士添加的Hanks' salt，以便在CO2培养箱外使用。McCoy的5A(改良)培养基需要补充血清，通常添加10%胎牛血清(FBS)。McCoy的5A(改良)培养基使用碳酸氢钠缓冲体系(2.2 g/L)，因此需要5-10%的CO2环境来维持生理pH值。
谨慎
不能用于人类或动物的治疗。超出预定用途的使用可能违反当地法律。
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McCoy's 5A (Modified) Medium B1136-060 |
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|
Components |
Molecular Weight |
Concentration (mg/L) |
mM |
|
Amino Acids |
|||
|
Glycine |
75.0 |
7.5 |
0.1 |
|
L-Alanine |
89.0 |
13.9 |
0.15617977 |
|
L-Arginine hydrochloride |
211.0 |
42.1 |
0.19952606 |
|
L-Asparagine |
132.0 |
45.0 |
0.3409091 |
|
L-Aspartic acid |
133.0 |
19.97 |
0.15015037 |
|
L-Cysteine |
121.0 |
31.5 |
0.2603306 |
|
L-Glutamic Acid |
147.0 |
22.1 |
0.15034014 |
|
L-Glutamine |
146.0 |
219.2 |
1.5013698 |
|
L-Histidine hydrochloride-H2O |
210.0 |
20.96 |
0.09980952 |
|
L-Hydroxyproline |
131.0 |
19.7 |
0.15038168 |
|
L-Isoleucine |
131.0 |
39.36 |
0.300458 |
|
L-Leucine |
131.0 |
39.36 |
0.300458 |
|
L-Lysine hydrochloride |
183.0 |
36.5 |
0.19945355 |
|
L-Methionine |
149.0 |
14.9 |
0.099999994 |
|
L-Phenylalanine |
165.0 |
16.5 |
0.1 |
|
L-Proline |
115.0 |
17.3 |
0.15043478 |
|
L-Serine |
105.0 |
26.3 |
0.25047618 |
|
L-Threonine |
119.0 |
17.9 |
0.15042016 |
|
L-Tryptophan |
204.0 |
3.1 |
0.0151960775 |
|
L-Tyrosine |
181.0 |
18.1 |
0.1 |
|
L-Valine |
117.0 |
17.6 |
0.15042736 |
|
Vitamins |
|||
|
Ascorbic Acid |
176.0 |
0.5 |
0.0028409092 |
|
Biotin |
244.0 |
0.2 |
8.1967213E-4 |
|
Choline chloride |
140.0 |
5.0 |
0.035714287 |
|
D-Calcium pantothenate |
477.0 |
0.2 |
4.1928721E-4 |
|
Folic Acid |
441.0 |
10.0 |
0.022675738 |
|
Niacinamide |
122.0 |
0.5 |
0.0040983604 |
|
Nicotinic acid (Niacin) |
123.0 |
0.5 |
0.0040650405 |
|
Para-Aminobenzoic Acid |
137.0 |
1.0 |
0.00729927 |
|
Pyridoxal hydrochloride |
204.0 |
0.5 |
0.0024509805 |
|
Pyridoxine hydrochloride |
206.0 |
0.5 |
0.0024271845 |
|
Riboflavin |
376.0 |
0.2 |
5.319149E-4 |
|
Thiamine hydrochloride |
337.0 |
0.2 |
5.934718E-4 |
|
Vitamin B12 |
1355.0 |
2.0 |
0.0014760147 |
|
i-Inositol |
180.0 |
36.0 |
0.2 |
|
Inorganic Salts |
|||
|
Calcium Chloride (CaCl2) (anhyd.) |
111.0 |
100.0 |
0.9009009 |
|
Magnesium Sulfate (MgSO4-7H2O) |
246.0 |
200.0 |
0.8130081 |
|
Potassium Chloride (KCl) |
75.0 |
400.0 |
5.3333335 |
|
Sodium Bicarbonate (NaHCO3) |
84.0 |
2200.0 |
26.190475 |
|
Sodium Chloride (NaCl) |
58.0 |
6460.0 |
111.37931 |
|
Sodium Phosphate monobasic (NaH2PO4-H2O) |
138.0 |
580.0 |
4.2028985 |
|
Other Components |
|||
|
Bacto-Peptone |
600.0 |
Infinity |
|
|
D-Glucose (Dextrose) |
180.0 |
3000.0 |
16.666666 |
|
Glutathione (reduced) |
307.0 |
0.5 |
0.0016286644 |
|
Phenol Red |
376.4 |
10.0 |
0.026567481 |