改良型的1640培养基，含HEPES
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- 产品描述
- COA
- 用户指南
- 配方
- 产品参数
|
货号 |
B1106-025 |
|
包含 |
酚红，HEPES，L-谷氨酰胺 |
|
不含 |
丙酮酸钠 |
|
PH |
7.4 |
|
外观 |
清澈的红色液体 |
|
规格 |
500ML |
|
单价(¥） |
90 |
|
Triple 0.1µm sterile-filtered |
|
|
储存条件 |
2°C to 8°C (避光保存) |
|
运输条件 |
室温 |
|
保质期 |
自生产日期起12个月 |
RPMI1640培养基最初用于培养人的白血病细胞悬浮和单层细胞。Roswell Park Memorial Institute (RPMI) 1640培养基已被发现适用于多种哺乳动物细胞，包括HeLa、Jurkat、MCF-7、PC12、PBMC、星形胶质细胞和癌。BIOEXPLORER®提供各种RPMI 1640培养基修饰，适用于各种细胞培养应用。RPMI 1640培养基独特于其他培养基，因为它含有还原剂谷胱甘肽和高浓度维生素。RPMI 1640培养基含有生物素、维生素B12和PABA，这些在Eagle's Minimal Essential培养基或Dulbecco'sModified Eagle培养基中没有的。此外，维生素肌醇和胆碱的浓度非常高。RPMI 1640培养基不含蛋白质、脂质或生长因子。因此，RPMI 1640培养基需要补充，通常添加10%胎牛血清(FBS)。RPMI 1640培养基采用碳酸氢钠缓冲体系(2.0 g/L)，因此需要5-10%的CO2环境来维持生理pH值。
谨慎
不能用于人类或动物的治疗。超出预定用途的使用可能违反当地法律。
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RPMI 1640 Medium, HEPES B1106-025 |
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Components |
Molecular Weight |
Concentration (mg/L) |
mM |
|
Amino Acids |
|||
|
Glycine |
75.0 |
10.0 |
0.13333334 |
|
L-Arginine |
174.0 |
200.0 |
1.1494253 |
|
L-Asparagine |
132.0 |
50.0 |
0.37878788 |
|
L-Aspartic acid |
133.0 |
20.0 |
0.15037593 |
|
L-Cystine 2HCl |
313.0 |
65.0 |
0.20766774 |
|
L-Glutamic Acid |
147.0 |
20.0 |
0.13605443 |
|
L-Glutamine |
146.0 |
300.0 |
2.0547945 |
|
L-Histidine |
155.0 |
15.0 |
0.09677419 |
|
L-Hydroxyproline |
131.0 |
20.0 |
0.15267175 |
|
L-Isoleucine |
131.0 |
50.0 |
0.3816794 |
|
L-Leucine |
131.0 |
50.0 |
0.3816794 |
|
L-Lysine hydrochloride |
146.0 |
40.0 |
0.2739726 |
|
L-Methionine |
149.0 |
15.0 |
0.10067114 |
|
L-Phenylalanine |
165.0 |
15.0 |
0.09090909 |
|
L-Proline |
115.0 |
20.0 |
0.17391305 |
|
L-Serine |
105.0 |
30.0 |
0.2857143 |
|
L-Threonine |
119.0 |
20.0 |
0.16806723 |
|
L-Tryptophan |
204.0 |
5.0 |
0.024509804 |
|
L-Tyrosine disodium salt dihydrate |
261.0 |
29.0 |
0.11111111 |
|
L-Valine |
117.0 |
20.0 |
0.17094018 |
|
Vitamins |
|||
|
Biotin |
244.0 |
0.2 |
8.1967213E-4 |
|
Choline chloride |
140.0 |
3.0 |
0.021428572 |
|
D-Calcium pantothenate |
477.0 |
0.25 |
5.24109E-4 |
|
Folic Acid |
441.0 |
1.0 |
0.0022675737 |
|
Niacinamide |
122.0 |
1.0 |
0.008196721 |
|
Para-Aminobenzoic Acid |
137.0 |
1.0 |
0.00729927 |
|
Pyridoxine hydrochloride |
206.0 |
1.0 |
0.004854369 |
|
Riboflavin |
376.0 |
0.2 |
5.319149E-4 |
|
Thiamine hydrochloride |
337.0 |
1.0 |
0.002967359 |
|
Vitamin B12 |
1355.0 |
0.005 |
3.690037E-6 |
|
i-Inositol |
180.0 |
35.0 |
0.19444445 |
|
Inorganic Salts |
|||
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Calcium nitrate (Ca(NO3)2 4H2O) |
236.0 |
100.0 |
0.42372882 |
|
Magnesium Sulfate (MgSO4) (anhyd.) |
120.0 |
48.84 |
0.407 |
|
Potassium Chloride (KCl) |
75.0 |
400.0 |
5.3333335 |
|
Sodium Bicarbonate (NaHCO3) |
84.0 |
2000.0 |
23.809525 |
|
Sodium Chloride (NaCl) |
58.0 |
5300.0 |
91.37931 |
|
Sodium Phosphate dibasic (Na2HPO4) anhydrous |
142.0 |
800.0 |
5.633803 |
|
Other Components |
|||
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D-Glucose (Dextrose) |
180.0 |
2000.0 |
11.111111 |
|
Glutathione (reduced) |
307.0 |
1.0 |
0.003257329 |
|
HEPES |
238.0 |
5958.0 |
25.033613 |
|
Phenol Red |
376.4 |
5.0 |
0.013283741 |