DMEM, High Glucose,HEPES, no Pyruvate
- Detail
- Description
- COA
- User Guide
- Formula
- Parameters
| Catalog Number | B1101-010 |
| With | High Glucose ,HEPES,L-Glutamine, Phenol Red |
| Without | Sodium Pyruvate |
| Appearance | Clear reddish solution |
| PH | 7.4 |
| Product Size | 500ML |
| Unit-Price($） | 11.3 |
| Triple 0.1µm sterile-filtered | |
| Storage conditions | 2°C to 8°C (protect from light) |
| Shipping conditions | Room Temperature |
| Shelf life | 12 months from date of manufacture |
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. BIOEXPLORER® offer a variety of DMEM modifications for a range of cell culture applications. Find the . DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM with Glutamine supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
CAUTION
Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
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DMEM, high glucose, HEPES B1101-010 |
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Components |
Molecular Weight |
Concentration (mg/L) |
mM |
|
Amino Acids |
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|
Glycine |
75.0 |
30.0 |
0.4 |
|
L-Arginine hydrochloride |
211.0 |
84.0 |
0.39810428 |
|
L-Cystine 2HCl |
313.0 |
63.0 |
0.20127796 |
|
L-Glutamine |
146.0 |
584.0 |
4.0 |
|
L-Histidine hydrochloride-H2O |
210.0 |
42.0 |
0.2 |
|
L-Isoleucine |
131.0 |
105.0 |
0.8015267 |
|
L-Leucine |
131.0 |
105.0 |
0.8015267 |
|
L-Lysine hydrochloride |
183.0 |
146.0 |
0.7978142 |
|
L-Methionine |
149.0 |
30.0 |
0.20134228 |
|
L-Phenylalanine |
165.0 |
66.0 |
0.4 |
|
L-Serine |
105.0 |
42.0 |
0.4 |
|
L-Threonine |
119.0 |
95.0 |
0.79831934 |
|
L-Tryptophan |
204.0 |
16.0 |
0.078431375 |
|
L-Tyrosine disodium salt dihydrate |
261.0 |
104.0 |
0.39846742 |
|
L-Valine |
117.0 |
94.0 |
0.8034188 |
|
Vitamins |
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|
Choline chloride |
140.0 |
4.0 |
0.028571429 |
|
D-Calcium pantothenate |
477.0 |
4.0 |
0.008385744 |
|
Folic Acid |
441.0 |
4.0 |
0.009070295 |
|
Niacinamide |
122.0 |
4.0 |
0.032786883 |
|
Pyridoxine hydrochloride |
206.0 |
4.0 |
0.019417476 |
|
Riboflavin |
376.0 |
0.4 |
0.0010638298 |
|
Thiamine hydrochloride |
337.0 |
4.0 |
0.011869436 |
|
i-Inositol |
180.0 |
7.2 |
0.04 |
|
Inorganic Salts |
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|
Calcium Chloride (CaCl2) (anhyd.) |
111.0 |
200.0 |
1.8018018 |
|
Ferric Nitrate (Fe(NO3)3"9H2O) |
404.0 |
0.1 |
2.4752476E-4 |
|
Magnesium Sulfate (MgSO4) (anhyd.) |
120.0 |
97.67 |
0.8139166 |
|
Potassium Chloride (KCl) |
75.0 |
400.0 |
5.3333335 |
|
Sodium Bicarbonate (NaHCO3) |
84.0 |
3700.0 |
44.04762 |
|
Sodium Chloride (NaCl) |
58.0 |
4750.0 |
81.89655 |
|
Sodium Phosphate monobasic (NaH2PO4-H2O) |
138.0 |
125.0 |
0.9057971 |
|
Other Components |
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D-Glucose (Dextrose) |
180.0 |
4500.0 |
25.0 |
|
HEPES |
238.0 |
5958.0 |
25.033613 |
|
Phenol Red |
376.4 |
15.0 |
0.039851222 |