BIOEXPLORER®
DMEM,High Glucose, GlutaSUPER, Pyruvate
  • Detail
  • Description
  • COA
  • User Guide
  • Formula
  • Parameters
Catalog Number B1101-014
With

High Glucose , Sodium Pyruvate,GlutaSUPER,

Phenol Red

Without HEPES
Appearance Clear reddish solution
PH 7.4
Product Size 500ML
Unit-Price($) 11.2
Triple 0.1µm sterile-filtered
Storage conditions 2°C to 8°C (protect from light)
Shipping conditions Room Temperature
Shelf life 12 months from date of manufacture


DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM with Glutamine supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10%   Fetal Bovine Serum   (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

CAUTION

Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.





DMEM, high glucose, GlutaMAX(TM), pyruvate   B1101-014

Components

Molecular Weight

Concentration (mg/L)

Mm

Amino Acids




Glycine

75.0

30.0

0.4

L-Alanyl-Glutamine

217.0

862.0

3.9723501

L-Arginine hydrochloride

211.0

84.0

0.39810428

L-Cystine 2HCl

313.0

63.0

0.20127796

L-Histidine hydrochloride-H2O

210.0

42.0

0.2

L-Isoleucine

131.0

105.0

0.8015267

L-Leucine

131.0

105.0

0.8015267

L-Lysine hydrochloride

183.0

146.0

0.7978142

L-Methionine

149.0

30.0

0.20134228

L-Phenylalanine

165.0

66.0

0.4

L-Serine

105.0

42.0

0.4

L-Threonine

119.0

95.0

0.79831934

L-Tryptophan

204.0

16.0

0.078431375

L-Tyrosine disodium salt   dihydrate

261.0

104.0

0.39846742

L-Valine

117.0

94.0

0.8034188





Vitamins




Choline chloride

140.0

4.0

0.028571429

D-Calcium   pantothenate

477.0

4.0

0.008385744

Folic Acid

441.0

4.0

0.009070295

Niacinamide

122.0

4.0

0.032786883

Pyridoxine hydrochloride

206.0

4.0

0.019417476

Riboflavin

376.0

0.4

0.0010638298

Thiamine hydrochloride

376.0

4.0

0.011869436

i-Inositol

180.0

7.2

0.04





Inorganic Salts




Calcium Chloride (CaCl2) (anhyd.)   

111.0

200.0

1.8018018

Ferric Nitrate   (Fe(NO3)3"9H2O)

404.0

0.1

2.4752476E-4

Magnesium   Sulfate (MgSO4) (anhyd.)

120.0

97.67

0.8139166

Potassium Chloride (KCl)

75.0

400.0

5.3333335

Sodium Bicarbonate (NaHCO3)

84.0

3700.0

44.04762

Sodium Chloride (NaCl)

58.0

6400.0

110.344826

Sodium Phosphate monobasic   (NaH2PO4-H2O)

138.0

125.0

0.9057971





Other Components




D-Glucose (Dextrose)

180.0

4500.0

25

Phenol Red

376.4

15.0

0.039851222

Sodium Pyruvate

110.0

110.0

1.0



Price: $11.20
Catalog Number: B1101-014
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