Medium 199 ,HEPES
- Detail
- Description
- COA
- User Guide
- Formula
- Parameters
| Catalog Number | B1135-059 |
| With | L-glutamine, Phenol Red，HEPES |
| Without | Sodium Pyruvate |
| Appearance | Clear reddish solution |
| Product Size | 500ML |
| PH | 7.4 |
| Unit-Price($） | 12.5 |
| Triple 0.1µm sterile-filtered | |
| Storage conditions | 2°C to 8°C (protect from light) |
| Shipping conditions | Room Temperature |
| Shelf life | 12 months from date of manufacture |
Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. BIOEXPLORER®offer a variety of Medium 199 modifications for a range of cell culture applications. Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
CAUTION
Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
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Medium 199, Earle's Salts B1135-059 |
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Components |
Molecular Weight |
Concentration (mg/L) |
mM |
|
Amino Acids |
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|
Glycine |
75.0 |
50.0 |
0.6666667 |
|
L-Alanine |
89.0 |
25.0 |
0.28089887 |
|
L-Arginine hydrochloride |
211.0 |
70.0 |
0.33175355 |
|
L-Aspartic acid |
133.0 |
30.0 |
0.22556391 |
|
L-Cysteine hydrochloride-H2O |
176.0 |
0.1 |
5.681818E-4 |
|
L-Cystine 2HCl |
240.0 |
26.0 |
0.108333334 |
|
L-Glutamic Acid |
147.0 |
75.0 |
0.5102041 |
|
L-Glutamine |
146.0 |
100.0 |
0.6849315 |
|
L-Histidine hydrochloride-H2O |
210.0 |
21.88 |
0.10419047 |
|
L-Hydroxyproline |
131.0 |
10.0 |
0.07633588 |
|
L-Isoleucine |
131.0 |
40.0 |
0.3053435 |
|
L-Leucine |
131.0 |
60.0 |
0.45801526 |
|
L-Lysine hydrochloride |
183.0 |
70.0 |
0.38251367 |
|
L-Methionine |
149.0 |
15.0 |
0.10067114 |
|
L-Phenylalanine |
165.0 |
25.0 |
0.15151516 |
|
L-Proline |
115.0 |
40.0 |
0.3478261 |
|
L-Serine |
105.0 |
25.0 |
0.23809524 |
|
L-Threonine |
119.0 |
30.0 |
0.25210086 |
|
L-Tryptophan |
204.0 |
10.0 |
0.04901961 |
|
L-Tyrosine disodium salt dihydrate |
261.0 |
58.0 |
0.22222222 |
|
L-Valine |
117.0 |
25.0 |
0.21367522 |
|
Vitamins |
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|
Ascorbic Acid |
176.0 |
0.05 |
2.840909E-4 |
|
Biotin |
244.0 |
0.01 |
4.0983607E-5 |
|
Choline chloride |
140.0 |
0.5 |
0.0035714286 |
|
D-Calcium pantothenate |
477.0 |
0.01 |
2.096436E-5 |
|
Folic Acid |
441.0 |
0.01 |
2.2675737E-5 |
|
Menadione (Vitamin K3) |
172.0 |
0.01 |
5.8139532E-5 |
|
Niacinamide |
122.0 |
0.025 |
2.0491803E-4 |
|
Nicotinic acid (Niacin) |
123.0 |
0.025 |
2.0325204E-4 |
|
Para-Aminobenzoic Acid |
137.0 |
0.05 |
3.6496352E-4 |
|
Pyridoxal hydrochloride |
204.0 |
0.025 |
1.2254903E-4 |
|
Pyridoxine hydrochloride |
206.0 |
0.025 |
1.21359226E-4 |
|
Riboflavin |
376.0 |
0.01 |
2.6595744E-5 |
|
Thiamine hydrochloride |
337.0 |
0.01 |
2.967359E-5 |
|
Vitamin A (acetate) |
328.0 |
0.1 |
3.0487805E-4 |
|
Vitamin D2 (Calciferol) |
397.0 |
0.1 |
2.5188917E-4 |
|
alpha Tocopherol phos. Na salt |
554.7 |
0.01 |
1.8027762E-5 |
|
i-Inositol |
180.0 |
0.05 |
2.7777778E-4 |
|
Inorganic Salts |
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|
Calcium Chloride (CaCl2) (anhyd.) |
111.0 |
200.0 |
1.8018018 |
|
Ferric nitrate (Fe(NO3)-9H2O) |
404.0 |
0.7 |
0.0017326733 |
|
Magnesium Sulfate (MgSO4) (anhyd.) |
120.0 |
97.67 |
0.8139166 |
|
Potassium Chloride (KCl) |
75.0 |
400.0 |
5.3333335 |
|
Sodium Bicarbonate (NaHCO3) |
84.0 |
2200.0 |
26.190475 |
|
Sodium Chloride (NaCl) |
58.0 |
6800.0 |
117.24138 |
|
Sodium Phosphate monobasic (NaH2PO4-H2O) |
138.0 |
140.0 |
1.0144928 |
|
Other Components |
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|
2-deoxy-D-ribose |
134.0 |
0.5 |
0.0037313432 |
|
Adenine sulfate |
404.0 |
10.0 |
0.024752475 |
|
Adenosine 5'-phosphate |
347.0 |
0.2 |
5.763689E-4 |
|
Adenosine 5'-triphosphate |
605.0 |
1.0 |
0.0016528926 |
|
Cholesterol |
387.0 |
0.2 |
5.1679584E-4 |
|
D-Glucose (Dextrose) |
180.0 |
1000.0 |
5.5555553 |
|
Glutathione (reduced) |
307.0 |
0.05 |
1.6286645E-4 |
|
Guanine hydrochloride |
188.0 |
0.3 |
0.0015957447 |
|
Hypoxanthine Na |
136.0 |
0.4 |
0.0029411765 |
|
Phenol Red |
376.4 |
20.0 |
0.053134963 |
|
Ribose |
150.0 |
0.5 |
0.0033333334 |
|
Sodium Acetate |
82.0 |
50.0 |
0.6097561 |
|
Thymine |
126.0 |
0.3 |
0.0023809525 |
|
Tween 80® |
20.0 |
Infinity |
|
|
Uracil |
112.0 |
0.3 |
0.0026785715 |
|
Xanthine-Na |
152.0 |
0.34 |
0.0022368422 |