McCoy's5A Medium
- Detail
- Description
- COA
- User Guide
- Formula
- Parameters
| Catalog Number | B1136-060 |
|
With |
L-Glutamine，High Glucose， Bacto-peptone，Phenol Red |
| Without | Sodium Pyruvate，HEPES |
| Appearance | Clear reddish solution |
| Product Size | 500ML |
| Unit-Price($） | 12.5 |
| Triple 0.1µm sterile-filtered | |
| Storage conditions | 2°C to 8°C (protect from light) |
| Shipping conditions | Room Temperature |
| Shelf life | 12 months from date of manufacture |
McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues.
Using McCoy's 5A (modified) Medium,Dr. Thomas McCoy originally formulated McCoy's 5A medium as a modification of Basal Medium 5A. Unlike other media, McCoy's 5A contains the reducing agent glutathione, bacto-peptone, and a high level of glucose. This product also includes Dr. Hsu's addition of Hanks' salts to enable use outside a CO2 incubator. McCoy's 5A (modified) Medium requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS). McCoy's 5A (modified) Medium uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
CAUTION
Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
|
McCoy's 5A (Modified) Medium B1136-060 |
|||
|
Components |
Molecular Weight |
Concentration (mg/L) |
mM |
|
Amino Acids |
|||
|
Glycine |
75.0 |
7.5 |
0.1 |
|
L-Alanine |
89.0 |
13.9 |
0.15617977 |
|
L-Arginine hydrochloride |
211.0 |
42.1 |
0.19952606 |
|
L-Asparagine |
132.0 |
45.0 |
0.3409091 |
|
L-Aspartic acid |
133.0 |
19.97 |
0.15015037 |
|
L-Cysteine |
121.0 |
31.5 |
0.2603306 |
|
L-Glutamic Acid |
147.0 |
22.1 |
0.15034014 |
|
L-Glutamine |
146.0 |
219.2 |
1.5013698 |
|
L-Histidine hydrochloride-H2O |
210.0 |
20.96 |
0.09980952 |
|
L-Hydroxyproline |
131.0 |
19.7 |
0.15038168 |
|
L-Isoleucine |
131.0 |
39.36 |
0.300458 |
|
L-Leucine |
131.0 |
39.36 |
0.300458 |
|
L-Lysine hydrochloride |
183.0 |
36.5 |
0.19945355 |
|
L-Methionine |
149.0 |
14.9 |
0.099999994 |
|
L-Phenylalanine |
165.0 |
16.5 |
0.1 |
|
L-Proline |
115.0 |
17.3 |
0.15043478 |
|
L-Serine |
105.0 |
26.3 |
0.25047618 |
|
L-Threonine |
119.0 |
17.9 |
0.15042016 |
|
L-Tryptophan |
204.0 |
3.1 |
0.0151960775 |
|
L-Tyrosine |
181.0 |
18.1 |
0.1 |
|
L-Valine |
117.0 |
17.6 |
0.15042736 |
|
Vitamins |
|||
|
Ascorbic Acid |
176.0 |
0.5 |
0.0028409092 |
|
Biotin |
244.0 |
0.2 |
8.1967213E-4 |
|
Choline chloride |
140.0 |
5.0 |
0.035714287 |
|
D-Calcium pantothenate |
477.0 |
0.2 |
4.1928721E-4 |
|
Folic Acid |
441.0 |
10.0 |
0.022675738 |
|
Niacinamide |
122.0 |
0.5 |
0.0040983604 |
|
Nicotinic acid (Niacin) |
123.0 |
0.5 |
0.0040650405 |
|
Para-Aminobenzoic Acid |
137.0 |
1.0 |
0.00729927 |
|
Pyridoxal hydrochloride |
204.0 |
0.5 |
0.0024509805 |
|
Pyridoxine hydrochloride |
206.0 |
0.5 |
0.0024271845 |
|
Riboflavin |
376.0 |
0.2 |
5.319149E-4 |
|
Thiamine hydrochloride |
337.0 |
0.2 |
5.934718E-4 |
|
Vitamin B12 |
1355.0 |
2.0 |
0.0014760147 |
|
i-Inositol |
180.0 |
36.0 |
0.2 |
|
Inorganic Salts |
|||
|
Calcium Chloride (CaCl2) (anhyd.) |
111.0 |
100.0 |
0.9009009 |
|
Magnesium Sulfate (MgSO4-7H2O) |
246.0 |
200.0 |
0.8130081 |
|
Potassium Chloride (KCl) |
75.0 |
400.0 |
5.3333335 |
|
Sodium Bicarbonate (NaHCO3) |
84.0 |
2200.0 |
26.190475 |
|
Sodium Chloride (NaCl) |
58.0 |
6460.0 |
111.37931 |
|
Sodium Phosphate monobasic (NaH2PO4-H2O) |
138.0 |
580.0 |
4.2028985 |
|
Other Components |
|||
|
Bacto-Peptone |
600.0 |
Infinity |
|
|
D-Glucose (Dextrose) |
180.0 |
3000.0 |
16.666666 |
|
Glutathione (reduced) |
307.0 |
0.5 |
0.0016286644 |
|
Phenol Red |
376.4 |
10.0 |
0.026567481 |