BIOEXPLORER®
MEM Alpha Modification no Nucleosides Medium
  • Detail
  • Description
  • COA
  • User Guide
  • Formula
  • Parameters
Catalog Number B1131-056
With L-Glutamine, Phenol Red
Without HEPES,   Ribonucleosides,Deoxyribonucleosides
Appearance Clear reddish solution
Product Size 500ML
PH 7.4
Unit-Price($) 12.2
Triple 0.1µm sterile-filtered
Storage conditions 2°C to 8°C (protect from light)
Shipping conditions Room Temperature
Shelf life: 12months from date of manufacture

MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. BIOEXPLORER® offer a variety of MEM α modifications for a range of cell culture applications. MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR-negative cells. This product is made with Earle's salts. MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

CAUTION

Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.





MEM α, no nucleosides   B1131-056 

Components

Molecular Weight

Concentration (mg/L)

mM

Amino   Acids

Glycine

75.0 

50.0 

0.6666667

L-Alanine

89.0 

25.0 

0.28089887

L-Arginine   hydrochloride

211.0 

126.98 

0.601801

L-Asparagine-H2O

150.0 

50.0 

0.33333334

L-Aspartic   acid

133.0 

30.0 

0.22556391

L-Cysteine   hydrochloride-H2O

176.0 

100.0 

0.5681818

L-Cystine   2HCl

313.0 

31.0 

0.09904154

L-Glutamic   Acid

147.0 

75.0 

0.5102041

L-Glutamine

146.0 

292.0 

2.0 

L-Histidine   hydrochloride-H2O

210.0 

42.0 

0.2

L-Isoleucine

131.0 

52.4 

0.4

L-Leucine

131.0 

52.0 

0.39694658

L-Lysine   hydrochloride

183.0 

73.0 

0.3989071

L-Methionine

149.0 

15.0 

0.10067114

L-Phenylalanine

165.0 

32.0 

0.19393939

L-Proline

115.0 

40.0 

0.3478261

L-Serine

105.0 

25.0 

0.23809524

L-Threonine

119.0 

48.0 

0.40336135

L-Tryptophan

204.0 

10.0 

0.04901961

L-Tyrosine   disodium salt

262.0 

52.0 

0.19847329

L-Valine

117.0 

46.0 

0.3931624

Vitamins

Ascorbic   Acid

176.0 

50.0 

0.2840909

Biotin

244.0 

0.1 

4.0983607E-4

Choline   chloride

140.0 

1.0 

0.007142857

D-Calcium   pantothenate

477.0 

1.0 

0.002096436

Folic   Acid

441.0 

1.0 

0.0022675737 

Niacinamide

122.0 

1.0 

0.008196721

Pyridoxal   hydrochloride

204.0 

1.0 

0.004901961

Riboflavin

376.0 

0.1 

2.6595744E-4

Thiamine   hydrochloride

337.0 

1.0 

0.002967359

Vitamin   B12

1355.0 

1.36 

0.0010036901 

i-Inositol

180.0 

2.0 

0.011111111

Inorganic   Salts

Calcium   Chloride (CaCl2) (anhyd.)

111.0 

200.0 

1.8018018

Magnesium   Sulfate (MgSO4) (anhyd.)

120.0 

97.67 

0.8139166

Potassium   Chloride (KCl)

75.0 

400.0 

5.3333335

Sodium   Bicarbonate (NaHCO3)

84.0 

2200.0 

26.190475

Sodium   Chloride (NaCl)

58.0 

6800.0 

117.24138

Sodium   Phosphate monobasic (NaH2PO4-H2O)

138.0 

140.0 

1.0144928

Other   Components

D-Glucose   (Dextrose)

180.0 

1000.0 

5.5555553

Lipoic   Acid

206.0 

0.2 

9.708738E-4

Phenol   Red

376.4 

10.0 

0.026567481

Sodium   Pyruvate

110.0 

110.0 

1.0 



Price: $12.20
Catalog Number: B1131-056
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