MEM Alpha Modification no Nucleosides Medium
- Detail
- Description
- COA
- User Guide
- Formula
- Parameters
| Catalog Number | B1131-056 |
| With | L-Glutamine, Phenol Red |
| Without | HEPES, Ribonucleosides,Deoxyribonucleosides |
| Appearance | Clear reddish solution |
| Product Size | 500ML |
| PH | 7.4 |
| Unit-Price($） | 12.2 |
| Triple 0.1µm sterile-filtered | |
| Storage conditions | 2°C to 8°C (protect from light) |
| Shipping conditions | Room Temperature |
| Shelf life: | 12months from date of manufacture |
MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. BIOEXPLORER® offer a variety of MEM α modifications for a range of cell culture applications. MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR-negative cells. This product is made with Earle's salts. MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
CAUTION
Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
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MEM α, no nucleosides B1131-056 |
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Components |
Molecular Weight |
Concentration (mg/L) |
mM |
|
Amino Acids |
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|
Glycine |
75.0 |
50.0 |
0.6666667 |
|
L-Alanine |
89.0 |
25.0 |
0.28089887 |
|
L-Arginine hydrochloride |
211.0 |
126.98 |
0.601801 |
|
L-Asparagine-H2O |
150.0 |
50.0 |
0.33333334 |
|
L-Aspartic acid |
133.0 |
30.0 |
0.22556391 |
|
L-Cysteine hydrochloride-H2O |
176.0 |
100.0 |
0.5681818 |
|
L-Cystine 2HCl |
313.0 |
31.0 |
0.09904154 |
|
L-Glutamic Acid |
147.0 |
75.0 |
0.5102041 |
|
L-Glutamine |
146.0 |
292.0 |
2.0 |
|
L-Histidine hydrochloride-H2O |
210.0 |
42.0 |
0.2 |
|
L-Isoleucine |
131.0 |
52.4 |
0.4 |
|
L-Leucine |
131.0 |
52.0 |
0.39694658 |
|
L-Lysine hydrochloride |
183.0 |
73.0 |
0.3989071 |
|
L-Methionine |
149.0 |
15.0 |
0.10067114 |
|
L-Phenylalanine |
165.0 |
32.0 |
0.19393939 |
|
L-Proline |
115.0 |
40.0 |
0.3478261 |
|
L-Serine |
105.0 |
25.0 |
0.23809524 |
|
L-Threonine |
119.0 |
48.0 |
0.40336135 |
|
L-Tryptophan |
204.0 |
10.0 |
0.04901961 |
|
L-Tyrosine disodium salt |
262.0 |
52.0 |
0.19847329 |
|
L-Valine |
117.0 |
46.0 |
0.3931624 |
|
Vitamins |
|||
|
Ascorbic Acid |
176.0 |
50.0 |
0.2840909 |
|
Biotin |
244.0 |
0.1 |
4.0983607E-4 |
|
Choline chloride |
140.0 |
1.0 |
0.007142857 |
|
D-Calcium pantothenate |
477.0 |
1.0 |
0.002096436 |
|
Folic Acid |
441.0 |
1.0 |
0.0022675737 |
|
Niacinamide |
122.0 |
1.0 |
0.008196721 |
|
Pyridoxal hydrochloride |
204.0 |
1.0 |
0.004901961 |
|
Riboflavin |
376.0 |
0.1 |
2.6595744E-4 |
|
Thiamine hydrochloride |
337.0 |
1.0 |
0.002967359 |
|
Vitamin B12 |
1355.0 |
1.36 |
0.0010036901 |
|
i-Inositol |
180.0 |
2.0 |
0.011111111 |
|
Inorganic Salts |
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|
Calcium Chloride (CaCl2) (anhyd.) |
111.0 |
200.0 |
1.8018018 |
|
Magnesium Sulfate (MgSO4) (anhyd.) |
120.0 |
97.67 |
0.8139166 |
|
Potassium Chloride (KCl) |
75.0 |
400.0 |
5.3333335 |
|
Sodium Bicarbonate (NaHCO3) |
84.0 |
2200.0 |
26.190475 |
|
Sodium Chloride (NaCl) |
58.0 |
6800.0 |
117.24138 |
|
Sodium Phosphate monobasic (NaH2PO4-H2O) |
138.0 |
140.0 |
1.0144928 |
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Other Components |
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D-Glucose (Dextrose) |
180.0 |
1000.0 |
5.5555553 |
|
Lipoic Acid |
206.0 |
0.2 |
9.708738E-4 |
|
Phenol Red |
376.4 |
10.0 |
0.026567481 |
|
Sodium Pyruvate |
110.0 |
110.0 |
1.0 |