BIOEXPLORER®
RPMI1640 Medium,HEPES
  • Detail
  • Description
  • COA
  • User Guide
  • Formula
  • Parameters


Catalog Number B1106-025

With

Phenol Red,HEPES,L-Glutamine

Without Sodium pyruvate
PH 7.4
Appearance Clear reddish solution
Product Size 500ML
Unit-Price($) 11.2
Triple 0.1µm sterile-filtered
Storage conditions 2°C to 8°C (protect from light)
Shipping conditions Room Temperature
Shelf life 12 months from date of manufacture

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. BIOEXPLORER® offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications. RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

CAUTION

Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.





RPMI 1640 Medium, HEPES    B1106-025

Components

Molecular Weight

Concentration (mg/L)

mM

Amino   Acids

Glycine

75.0

10.0

0.13333334

L-Arginine

174.0

200.0

1.1494253

L-Asparagine

132.0

50.0

0.37878788

L-Aspartic   acid

133.0

20.0

0.15037593

L-Cystine   2HCl

313.0

65.0

0.20766774

L-Glutamic   Acid

147.0

20.0

0.13605443

L-Glutamine

146.0

300.0

2.0547945

L-Histidine

155.0

15.0

0.09677419

L-Hydroxyproline

131.0

20.0

0.15267175

L-Isoleucine

131.0

50.0

0.3816794

L-Leucine

131.0

50.0

0.3816794

L-Lysine   hydrochloride

146.0

40.0

0.2739726

L-Methionine

149.0

15.0

0.10067114

L-Phenylalanine

165.0

15.0

0.09090909

L-Proline

115.0

20.0

0.17391305

L-Serine

105.0

30.0

0.2857143

L-Threonine

119.0

20.0

0.16806723

L-Tryptophan

204.0

5.0

0.024509804

L-Tyrosine   disodium salt dihydrate

261.0

29.0

0.11111111

L-Valine

117.0

20.0

0.17094018

Vitamins

Biotin

244.0

0.2

8.1967213E-4

Choline   chloride

140.0

3.0

0.021428572

D-Calcium   pantothenate

477.0

0.25

5.24109E-4

Folic   Acid

441.0

1.0

0.0022675737

Niacinamide

122.0

1.0

0.008196721

Para-Aminobenzoic   Acid

137.0

1.0

0.00729927

Pyridoxine   hydrochloride

206.0

1.0

0.004854369

Riboflavin

376.0

0.2

5.319149E-4

Thiamine   hydrochloride

337.0

1.0

0.002967359

Vitamin   B12

1355.0

0.005

3.690037E-6

i-Inositol

180.0

35.0

0.19444445

Inorganic   Salts

Calcium   nitrate (Ca(NO3)2 4H2O)

236.0

100.0

0.42372882

Magnesium   Sulfate (MgSO4) (anhyd.)

120.0

48.84

0.407

Potassium   Chloride (KCl)

75.0

400.0

5.3333335

Sodium   Bicarbonate (NaHCO3)

84.0

2000.0

23.809525

Sodium   Chloride (NaCl)

58.0

5300.0

91.37931

Sodium   Phosphate dibasic (Na2HPO4) anhydrous

142.0

800.0

5.633803

Other   Components

D-Glucose   (Dextrose)

180.0

2000.0

11.111111

Glutathione   (reduced)

307.0

1.0

0.003257329

HEPES

238.0

5958.0

25.033613

Phenol   Red

376.4

5.0

0.013283741



Price: $11.20
Catalog Number: B1106-025
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