RPMI1640 Medium,HEPES
- Detail
- Description
- COA
- User Guide
- Formula
- Parameters
| Catalog Number | B1106-025 |
|
With |
Phenol Red,HEPES,L-Glutamine |
| Without | Sodium pyruvate |
| PH | 7.4 |
| Appearance | Clear reddish solution |
| Product Size | 500ML |
| Unit-Price($） | 11.2 |
| Triple 0.1µm sterile-filtered | |
| Storage conditions | 2°C to 8°C (protect from light) |
| Shipping conditions | Room Temperature |
| Shelf life | 12 months from date of manufacture |
RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. BIOEXPLORER® offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications. RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
CAUTION
Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
|
RPMI 1640 Medium, HEPES B1106-025 |
|||
|
Components |
Molecular Weight |
Concentration (mg/L) |
mM |
|
Amino Acids |
|||
|
Glycine |
75.0 |
10.0 |
0.13333334 |
|
L-Arginine |
174.0 |
200.0 |
1.1494253 |
|
L-Asparagine |
132.0 |
50.0 |
0.37878788 |
|
L-Aspartic acid |
133.0 |
20.0 |
0.15037593 |
|
L-Cystine 2HCl |
313.0 |
65.0 |
0.20766774 |
|
L-Glutamic Acid |
147.0 |
20.0 |
0.13605443 |
|
L-Glutamine |
146.0 |
300.0 |
2.0547945 |
|
L-Histidine |
155.0 |
15.0 |
0.09677419 |
|
L-Hydroxyproline |
131.0 |
20.0 |
0.15267175 |
|
L-Isoleucine |
131.0 |
50.0 |
0.3816794 |
|
L-Leucine |
131.0 |
50.0 |
0.3816794 |
|
L-Lysine hydrochloride |
146.0 |
40.0 |
0.2739726 |
|
L-Methionine |
149.0 |
15.0 |
0.10067114 |
|
L-Phenylalanine |
165.0 |
15.0 |
0.09090909 |
|
L-Proline |
115.0 |
20.0 |
0.17391305 |
|
L-Serine |
105.0 |
30.0 |
0.2857143 |
|
L-Threonine |
119.0 |
20.0 |
0.16806723 |
|
L-Tryptophan |
204.0 |
5.0 |
0.024509804 |
|
L-Tyrosine disodium salt dihydrate |
261.0 |
29.0 |
0.11111111 |
|
L-Valine |
117.0 |
20.0 |
0.17094018 |
|
Vitamins |
|||
|
Biotin |
244.0 |
0.2 |
8.1967213E-4 |
|
Choline chloride |
140.0 |
3.0 |
0.021428572 |
|
D-Calcium pantothenate |
477.0 |
0.25 |
5.24109E-4 |
|
Folic Acid |
441.0 |
1.0 |
0.0022675737 |
|
Niacinamide |
122.0 |
1.0 |
0.008196721 |
|
Para-Aminobenzoic Acid |
137.0 |
1.0 |
0.00729927 |
|
Pyridoxine hydrochloride |
206.0 |
1.0 |
0.004854369 |
|
Riboflavin |
376.0 |
0.2 |
5.319149E-4 |
|
Thiamine hydrochloride |
337.0 |
1.0 |
0.002967359 |
|
Vitamin B12 |
1355.0 |
0.005 |
3.690037E-6 |
|
i-Inositol |
180.0 |
35.0 |
0.19444445 |
|
Inorganic Salts |
|||
|
Calcium nitrate (Ca(NO3)2 4H2O) |
236.0 |
100.0 |
0.42372882 |
|
Magnesium Sulfate (MgSO4) (anhyd.) |
120.0 |
48.84 |
0.407 |
|
Potassium Chloride (KCl) |
75.0 |
400.0 |
5.3333335 |
|
Sodium Bicarbonate (NaHCO3) |
84.0 |
2000.0 |
23.809525 |
|
Sodium Chloride (NaCl) |
58.0 |
5300.0 |
91.37931 |
|
Sodium Phosphate dibasic (Na2HPO4) anhydrous |
142.0 |
800.0 |
5.633803 |
|
Other Components |
|||
|
D-Glucose (Dextrose) |
180.0 |
2000.0 |
11.111111 |
|
Glutathione (reduced) |
307.0 |
1.0 |
0.003257329 |
|
HEPES |
238.0 |
5958.0 |
25.033613 |
|
Phenol Red |
376.4 |
5.0 |
0.013283741 |