DMEM/F12, no HEPES Medium
- Detail
- Description
- COA
- User Guide
- Formula
- Parameters
| Catalog Number | B1121-042 |
| With | L-glutamine, Phenol Red,Sodium Pyruvate |
|
Without |
HEPES |
| Appearance | Clear reddish solution |
| Product Size | 500ML |
| PH | 7.4 |
| Unit-Price($） | 11.3 |
| Triple 0.1µm sterile-filtered | |
| Storage conditions | 2°C to 8°C (protect from light) |
| Shipping conditions | Room Temperature |
| Shelf life | 12 months from date of manufacture |
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. BIOEXPLORER® offer a variety of DMEM/F-12 modifications for a range of cell culture applications. DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH.
CAUTION
Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
|
DMEM/F-12 B1121-042 |
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Components |
Molecular Weight |
Concentration (mg/L) |
mM |
|
Amino Acids |
|||
|
Glycine |
75.0 |
18.75 |
0.25 |
|
L-Alanine |
89.0 |
4.45 |
0.049999997 |
|
L-Arginine hydrochloride |
211.0 |
147.5 |
0.69905216 |
|
L-Asparagine-H2O |
150.0 |
7.5 |
0.05 |
|
L-Aspartic acid |
133.0 |
6.65 |
0.05 |
|
L-Cysteine hydrochloride-H2O |
176.0 |
17.56 |
0.09977272 |
|
L-Cystine 2HCl |
313.0 |
31.29 |
0.09996805 |
|
L-Glutamic Acid |
147.0 |
7.35 |
0.05 |
|
L-Glutamine |
146.0 |
365.0 |
2.5 |
|
L-Histidine hydrochloride-H2O |
210.0 |
31.48 |
0.14990476 |
|
L-Isoleucine |
131.0 |
54.47 |
0.41580153 |
|
L-Leucine |
131.0 |
59.05 |
0.45076334 |
|
L-Lysine hydrochloride |
183.0 |
91.25 |
0.4986339 |
|
L-Methionine |
149.0 |
17.24 |
0.11570469 |
|
L-Phenylalanine |
165.0 |
35.48 |
0.2150303 |
|
L-Proline |
115.0 |
17.25 |
0.15 |
|
L-Serine |
105.0 |
26.25 |
0.25 |
|
L-Threonine |
119.0 |
53.45 |
0.44915968 |
|
L-Tryptophan |
204.0 |
9.02 |
0.04421569 |
|
L-Tyrosine disodium salt dihydrate |
261.0 |
55.79 |
0.21375479 |
|
L-Valine |
117.0 |
52.85 |
0.4517094 |
|
Vitamins |
|||
|
Biotin |
244.0 |
0.0035 |
1.4344263E-5 |
|
Choline chloride |
140.0 |
8.98 |
0.06414285 |
|
D-Calcium pantothenate |
477.0 |
2.24 |
0.0046960167 |
|
Folic Acid |
441.0 |
2.65 |
0.0060090707 |
|
Niacinamide |
122.0 |
2.02 |
0.016557377 |
|
Pyridoxine hydrochloride |
206.0 |
2.013 |
0.009771844 |
|
Riboflavin |
376.0 |
0.219 |
5.824468E-4 |
|
Thiamine hydrochloride |
337.0 |
2.17 |
0.0064391694 |
|
Vitamin B12 |
1355.0 |
0.68 |
5.0184503E-4 |
|
i-Inositol |
180.0 |
12.6 |
0.07 |
|
Inorganic Salts |
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|
Calcium Chloride (CaCl2) (anhyd.) |
111.0 |
116.6 |
1.0504504 |
|
Cupric sulfate (CuSO4-5H2O) |
250.0 |
0.0013 |
5.2E-6 |
|
Ferric Nitrate (Fe(NO3)3"9H2O) |
404.0 |
0.05 |
1.2376238E-4 |
|
Ferric sulfate (FeSO4-7H2O) |
278.0 |
0.417 |
0.0015 |
|
Magnesium Chloride (anhydrous) |
95.0 |
28.64 |
0.30147368 |
|
Magnesium Sulfate (MgSO4) (anhyd.) |
120.0 |
48.84 |
0.407 |
|
Potassium Chloride (KCl) |
75.0 |
311.8 |
4.1573334 |
|
Sodium Bicarbonate (NaHCO3) |
84.0 |
2438.0 |
29.02381 |
|
Sodium Chloride (NaCl) |
58.0 |
6995.5 |
120.61207 |
|
Sodium Phosphate dibasic (Na2HPO4) anhydrous |
142.0 |
71.02 |
0.50014085 |
|
Sodium Phosphate monobasic (NaH2PO4-H2O) |
138.0 |
62.5 |
0.45289856 |
|
Zinc sulfate (ZnSO4-7H2O) |
288.0 |
0.432 |
0.0015 |
|
Other Components |
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|
D-Glucose (Dextrose) |
180.0 |
3151.0 |
17.505556 |
|
Hypoxanthine Na |
159.0 |
2.39 |
0.015031448 |
|
Linoleic Acid |
280.0 |
0.042 |
1.4999999E-4 |
|
Lipoic Acid |
206.0 |
0.105 |
5.097087E-4 |
|
Phenol Red |
376.4 |
8.1 |
0.021519661 |
|
Putrescine 2HCl |
161.0 |
0.081 |
5.031056E-4 |
|
Sodium Pyruvate |
110.0 |
55.0 |
0.5 |
|
Thymidine |
242.0 |
0.365 |
0.0015082645 |