Basal Medium Eagle (BME)
- Detail
- Description
- COA
- User Guide
- Formula
- Parameters
| Catalog Number | B1150-114 |
| With | Phenol Red |
| Without | Sodium Pyruvate,HEPES,L-glutamine |
| Appearance | Clear reddish solution |
| Product Size | 500ML |
| Unit-Price($） | 22.0 |
| Triple 0.1µm sterile-filtered | |
| Storage conditions | 2°C to 8°C (protect from light) |
| Shipping conditions | Room Temperature |
| Shelf life | 12months from date of manufacture |
Basal Medium Eagle (BME) is a widely used synthetic basal medium for supporting the growth of many different mammalian cells. BME was originally developed by Harry Eagle for HeLa cells and mouse fibroblasts, when he discovered the minimum requirements for cell growth in vitro. BME has since been used for other human lines, including WI-38 and MRC-5.
BME contains eight B vitamins, the ten essential amino acids, plus cystine, tyrosine, and glutamine. There are a number of modifications of BME: Minimal Essential Medium, Glasgow's Medium and Dulbecco's Modified Eagle's Medium. This BME formulation contains Earle's salts for use in a CO2 incubator.
CAUTION
Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law.
|
Basal Medium Eagle (BME) B1150-114 |
|||
|
Components |
Molecular Weight |
Concentration (mg/L) |
mM |
|
Amino Acids |
|||
|
L-Arginine hydrochloride |
211.0 |
21.0 |
0.09952607 |
|
L-Cystine 2HCl |
313.0 |
16.0 |
0.05111821 |
|
L-Histidine |
155.0 |
8.0 |
0.051612902 |
|
L-Isoleucine |
131.0 |
26.0 |
0.19847329 |
|
L-Leucine |
131.0 |
26.0 |
0.19847329 |
|
L-Lysine hydrochloride |
183.0 |
36.47 |
0.19928962 |
|
L-Methionine |
149.0 |
7.5 |
0.05033557 |
|
L-Phenylalanine |
165.0 |
16.5 |
0.1 |
|
L-Threonine |
119.0 |
24.0 |
0.20168068 |
|
L-Tryptophan |
204.0 |
4.0 |
0.019607844 |
|
L-Tyrosine disodium salt dihydrate |
261.0 |
26.0 |
0.099616855 |
|
L-Valine |
117.0 |
23.5 |
0.2008547 |
|
Vitamins |
|||
|
Biotin |
244.0 |
1.0 |
0.0040983604 |
|
Choline chloride |
140.0 |
1.0 |
0.007142857 |
|
D-Calcium pantothenate |
477.0 |
1.0 |
0.002096436 |
|
Folic Acid |
441.0 |
1.0 |
0.0022675737 |
|
Nicotinamide |
122.0 |
1.0 |
0.008196721 |
|
Pyridoxal hydrochloride |
204.0 |
1.0 |
0.004901961 |
|
Riboflavin |
376.0 |
0.1 |
2.6595744E-4 |
|
Thiamine hydrochloride |
337.0 |
1.0 |
0.002967359 |
|
i-Inositol |
180.0 |
2.0 |
0.011111111 |
|
Inorganic Salts |
|||
|
Calcium Chloride (CaCl2) (anhyd.) |
111.0 |
200.0 |
1.8018018 |
|
Magnesium Sulfate (MgSO4) (anhyd.) |
120.0 |
97.7 |
0.81416667 |
|
Potassium Chloride (KCl) |
75.0 |
400.0 |
5.3333335 |
|
Sodium Bicarbonate (NaHCO3) |
84.0 |
2200.0 |
26.190475 |
|
Sodium Chloride (NaCl) |
58.0 |
6800.0 |
117.24138 |
|
Sodium Phosphate monobasic (NaH2PO4-H2O) |
138.0 |
140.0 |
1.0144928 |
|
Other Components |
|||
|
D-Glucose (Dextrose) |
180.0 |
1000.0 |
5.5555553 |
|
Phenol Red |
376.4 |
10.0 |
0.026567481 |