Ham's F-12 Nutrient Mix
- Detail
- Description
- COA
- User Guide
- Formula
- Parameters
| Catalog Number | B1299-109 |
|
With |
L-Glutamine ,Sodium Pyruvate ,Phenol Red |
| Without | HEPES |
| Appearance | Clear reddish solution |
| PH |
7.7 |
| Product Size | 500ML |
| Unit-Price($） | 11.8 |
| Triple 0.1µm sterile-filtered | |
| Storage conditions | 2°C to 8°C (protect from light) |
| Shipping conditions | Room Temperature |
| Shelf life | 12 months from date of manufacture |
Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell planting of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. BIOEXPLORER® Life Scienses offers a variety of F-12 modifications for a range of cell culture applications.
Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-12 has led to a variety of improved formulations.
Product Intended UseFor in vitro diagnostic use.
CAUTION
Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
Ham's F-12 Nutrient Mix--- B1299-109
|
Components |
Molecular Weight |
Concentration (mg/L) |
mM |
|
Amino Acids |
|||
|
Glycine |
75.0 |
7.5 |
0.1 |
|
L-Alanine |
89.0 |
8.9 |
0.099999994 |
|
L-Arginine hydrochloride |
211.0 |
211.0 |
1.0 |
|
L-Asparagine-H2O |
150.0 |
15.0 |
0.1 |
|
L-Aspartic acid |
133.0 |
13.3 |
0.1 |
|
L-Cysteine hydrochloride-H2O |
176.0 |
35.12 |
0.19954544 |
|
L-Glutamic Acid |
147.0 |
14.7 |
0.1 |
|
L-Glutamine |
146.0 |
146.0 |
1.0 |
|
L-Histidine hydrochloride-H2O |
210.0 |
21.0 |
0.1 |
|
L-Isoleucine |
131.0 |
4.0 |
0.030534351 |
|
L-Leucine |
131.0 |
13.1 |
0.1 |
|
L-Lysine hydrochloride |
183.0 |
36.5 |
0.19945355 |
|
L-Methionine |
149.0 |
4.5 |
0.030201342 |
|
L-Phenylalanine |
165.0 |
5.0 |
0.030303031 |
|
L-Proline |
115.0 |
34.5 |
0.3 |
|
L-Serine |
105.0 |
10.5 |
0.1 |
|
L-Threonine |
119.0 |
11.9 |
0.099999994 |
|
L-Tryptophan |
204.0 |
2.04 |
0.01 |
|
L-Tyrosine |
181.0 |
5.4 |
0.029834256 |
|
L-Valine |
117.0 |
11.7 |
0.1 |
|
Vitamins |
|||
|
Biotin |
244.0 |
0.0073 |
2.9918034E-5 |
|
Choline chloride |
140.0 |
14.0 |
0.1 |
|
D-Calcium pantothenate |
477.0 |
0.5 |
0.001048218 |
|
Folic Acid |
441.0 |
1.3 |
0.0029478457 |
|
Niacinamide |
122.0 |
0.036 |
2.9508196E-4 |
|
Pyridoxine hydrochloride |
206.0 |
0.06 |
2.9126214E-4 |
|
Riboflavin |
376.0 |
0.037 |
9.8404256E-5 |
|
Thiamine hydrochloride |
337.0 |
0.3 |
8.9020777E-4 |
|
Vitamin B12 |
1355.0 |
1.4 |
0.0010332103 |
|
i-Inositol |
180.0 |
18.0 |
0.1 |
|
Inorganic Salts |
|||
|
Calcium Chloride (CaCl2-2H2O) |
147.0 |
44.0 |
0.2993197 |
|
Cupric sulfate (CuSO4-5H2O) |
250.0 |
0.0025 |
1.0E-5 |
|
Ferric sulfate (FeSO4-7H2O) |
278.0 |
0.834 |
0.003 |
|
Magnesium Chloride (MgCl2-6H2O) |
203.0 |
122.0 |
0.6009852 |
|
Potassium Chloride (KCl) |
75.0 |
223.6 |
2.9813335 |
|
Sodium Bicarbonate (NaHCO3) |
84.0 |
1176.0 |
14.0 |
|
Sodium Chloride (NaCl) |
58.0 |
7599.0 |
131.01724 |
|
Sodium Phosphate dibasic (Na2HPO4) anhydrous |
142.0 |
142.0 |
1.0 |
|
Zinc sulfate (ZnSO4-7H2O) |
288.0 |
0.863 |
0.0029965276 |
|
Other Components |
|||
|
D-Glucose (Dextrose) |
180.0 |
1802.0 |
10.011111 |
|
Hypoxanthine |
136.0 |
4.0 |
0.029411765 |
|
Linoleic Acid |
280.0 |
0.084 |
2.9999999E-4 |
|
Lipoic Acid |
206.0 |
0.21 |
0.0010194174 |
|
Phenol Red |
376.4 |
1.2 |
0.0031880978 |
|
Putrescine 2HCl |
161.0 |
0.161 |
0.001 |
|
Sodium Pyruvate |
110.0 |
110.0 |
1.0 |
|
Thymidine |
242.0 |
0.7 |
0.002892562 |