Medium 199
- Detail
- Description
- COA
- User Guide
- Formula
- Parameters
| Catalog Number | B1135-055 |
| With | L-glutamine, Phenol Red，L-Amino acids |
| Without | HEPES, Sodium Pyruvate |
| Appearance | Clear reddish solution |
| Product Size | 500ML |
| Unit-Price($） | 12.3 |
| Triple 0.1µm sterile-filtered | |
| Storage conditions | 2°C to 8°C (protect from light) |
| Shipping conditions | Room Temperature |
| Shelf life | 12 months from date of manufacture |
Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. BIOEXPLORER® offer a variety of Medium 199 modifications for a range of cell culture applications. Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.
CAUTION
Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
|
Medium 199 B1135-055 |
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|
Components |
Molecular Weight |
Concentration (mg/L) |
mM |
|
Amino Acids |
|||
|
Glycine |
75.0 |
50.0 |
0.6666667 |
|
L-Alanine |
89.0 |
25.0 |
0.28089887 |
|
L-Arginine hydrochloride |
211.0 |
70.0 |
0.33175355 |
|
L-Aspartic acid |
133.0 |
30.0 |
0.22556391 |
|
L-Cysteine hydrochloride-H2O |
176.0 |
0.1 |
5.681818E-4 |
|
L-Cystine 2HCl |
240.0 |
26.0 |
0.108333334 |
|
L-Glutamic Acid |
147.0 |
75.0 |
0.5102041 |
|
L-Glutamine |
146.0 |
100.0 |
0.6849315 |
|
L-Histidine hydrochloride-H2O |
210.0 |
21.88 |
0.10419047 |
|
L-Hydroxyproline |
131.0 |
10.0 |
0.07633588 |
|
L-Isoleucine |
131.0 |
40.0 |
0.3053435 |
|
L-Leucine |
131.0 |
60.0 |
0.45801526 |
|
L-Lysine hydrochloride |
183.0 |
70.0 |
0.38251367 |
|
L-Methionine |
149.0 |
15.0 |
0.10067114 |
|
L-Phenylalanine |
165.0 |
25.0 |
0.15151516 |
|
L-Proline |
115.0 |
40.0 |
0.3478261 |
|
L-Serine |
105.0 |
25.0 |
0.23809524 |
|
L-Threonine |
119.0 |
30.0 |
0.25210086 |
|
L-Tryptophan |
204.0 |
10.0 |
0.04901961 |
|
L-Tyrosine disodium salt dihydrate |
261.0 |
58.0 |
0.22222222 |
|
L-Valine |
117.0 |
25.0 |
0.21367522 |
|
Vitamins |
|||
|
Ascorbic Acid |
176.0 |
0.05 |
2.840909E-4 |
|
Biotin |
244.0 |
0.01 |
4.0983607E-5 |
|
Choline chloride |
140.0 |
0.5 |
0.0035714286 |
|
D-Calcium pantothenate |
477.0 |
0.01 |
2.096436E-5 |
|
Folic Acid |
441.0 |
0.01 |
2.2675737E-5 |
|
Menadione (Vitamin K3) |
172.0 |
0.01 |
5.8139532E-5 |
|
Niacinamide |
122.0 |
0.025 |
2.0491803E-4 |
|
Nicotinic acid (Niacin) |
123.0 |
0.025 |
2.0325204E-4 |
|
Para-Aminobenzoic Acid |
137.0 |
0.05 |
3.6496352E-4 |
|
Pyridoxal hydrochloride |
204.0 |
0.025 |
1.2254903E-4 |
|
Pyridoxine hydrochloride |
206.0 |
0.025 |
1.21359226E-4 |
|
Riboflavin |
376.0 |
0.01 |
2.6595744E-5 |
|
Thiamine hydrochloride |
337.0 |
0.01 |
2.967359E-5 |
|
Vitamin A (acetate) |
328.0 |
0.1 |
3.0487805E-4 |
|
Vitamin D2 (Calciferol) |
397.0 |
0.1 |
2.5188917E-4 |
|
alpha Tocopherol phos. Na salt |
554.7 |
0.01 |
1.8027762E-5 |
|
i-Inositol |
180.0 |
0.05 |
2.7777778E-4 |
|
Inorganic Salts |
|||
|
Calcium Chloride (CaCl2) (anhyd.) |
111.0 |
200.0 |
1.8018018 |
|
Ferric nitrate (Fe(NO3)-9H2O) |
404.0 |
0.7 |
0.001732673 |
|
Magnesium Sulfate (MgSO4) (anhyd.) |
120.0 |
97.67 |
0.8139166 |
|
Potassium Chloride (KCl) |
75.0 |
400.0 |
5.3333335 |
|
Sodium Bicarbonate (NaHCO3) |
84.0 |
2200.0 |
26.190475 |
|
Sodium Chloride (NaCl) |
58.0 |
6800.0 |
117.24138 |
|
Sodium Phosphate monobasic (NaH2PO4-H2O) |
138.0 |
140.0 |
1.0144928 |
|
Other Components |
|||
|
2-deoxy-D-ribose |
134.0 |
0.5 |
0.0037313432 |
|
Adenine sulfate |
404.0 |
10.0 |
0.024752475 |
|
Adenosine 5'-phosphate |
347.0 |
0.2 |
5.763689E-4 |
|
Adenosine 5'-triphosphate |
605.0 |
1.0 |
0.0016528926 |
|
Cholesterol |
387.0 |
0.2 |
5.1679584E-4 |
|
D-Glucose (Dextrose) |
180.0 |
1000.0 |
5.5555553 |
|
Glutathione (reduced) |
307.0 |
0.05 |
1.6286645E-4 |
|
Guanine hydrochloride |
188.0 |
0.3 |
0.0015957447 |
|
Hypoxanthine Na |
136.0 |
0.4 |
0.0029411765 |
|
Phenol Red |
376.4 |
20.0 |
0.053134963 |
|
Ribose |
150.0 |
0.5 |
0.0033333334 |
|
Sodium Acetate |
82.0 |
50.0 |
0.6097561 |
|
Thymine |
126.0 |
0.3 |
0.0023809525 |
|
Tween 80® |
20.0 |
Infinity |
|
|
Uracil |
112.0 |
0.3 |
0.0026785715 |
|
Xanthine-Na |
152.0 |
0.34 |
0.0022368422 |