TRoZol Reagent Total RNA Extraction Reagent
- Detail
- Description
- COA
- User Guide
- Formula
- Parameters
| Catalog Number | Product Size | Unit-price |
| B6004-010 | 100ML | ($）134.0 |
| B6004-020 | 200ML | ($）262.0 |
| Downstream Application |
Cloning,Northern Blotting, Nuclease Protection Assays, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction |
| Final Product |
Total RNA,Transcriptome RNA, micro RNA |
| High Throughput Compatibility | Not High Throughput-Compatible (Manual) |
| Isolation Technology | Organic Extraction |
| Purification Time | 1 hour |
| Sample Type (General) | Bacteria,Blood,Cells,Plant Samples,Tissue,Viral Samples,Yeast |
| Scale | Scaleable |
| Shipping Condition | Room Temperature |
|
Storage conditions |
Room Temperature(protect from light) |
| Shelf life |
24months from date of manufacture |
| Starting Material (Amount) | Up to 1 g tissue,Up to 1 x 10^7 cells |
BIOEXPLORER® TRoZol is a broad-spectrum total RNA extraction reagent. The experiment is quick and convenient to operate, the color is bright, easy to layer. This reagent has a wide range of applications. It can extract total RNA from animal tissues, plant materials, various microorganisms and cultured cells. This method has a good separation effect on a small number of tissues (50-100mg) and cells (5x106) as well as a large number of tissues (≧1g) and cells (>107). The sample is fully cleaved in TRoZol to maximize RNA integrity. After chloroform was added for centrifugation, the solution was divided into three layers: the upper colorless aqueous phase, the middle layer, and the lower red organic phase. RNA was distributed in the supernatant layer. After collecting the supernatant, total RNA can be recovered by isopropanol precipitation. The extracted total RNA had good integrity and was free of protein and DNA contamination, which could be used in various molecular biology routine experiments, such as RT-PCR, Real-time RT-PCR, Northern Blot, Dot Blot, In vitro Translation, etc.
BIOEXPLORER® TRoZol reagent can promote the precipitation of various RNA of different species and different molecular weight sizes. For example, RNA agarose gel electrophoresis from rat liver and ethyl bromide staining showed many discontinuous stripe of high molecular weight (mRNA and hnRNA components) between 7kb and 15kb, two dominant ribosomes -5kb (28S) -2kb (18S), and low molecular weight =RNA between 0.1 and 0.3 kb(tRNA, 5S). When the extracted RNA was diluted with TE, its A260/A280 ratio was 1.8. (note that if agarose gel electrophoresis is used, the position of 28S is about 2kb, and the position of 18S is about 1kb. The gel position varies greatly with different concentrations.)
Features
RNA, DNA and proteins were extracted from a single sample.
With excellent cracking capacity.
Suitable for many types of samples, such as tissues, cells, serum, viruses and bacteria.
Suitable for small samples (50-100mg tissue, 5x107 cells),
It is suitable for a large number of samples (≧1g tissue or ≧1x107 cells).
Maintain RNA integrity.
Reliable purified RNA can be obtained from a variety of samples.
High stability, easy to keep, long validity.
Efficient operation is simple and quick.
It is suitable for the extraction of multiple molecular targets.
CAUTION
Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
