BIOEXPLORER®
Ham's F-10 Nutrient Mix
  • Detail
  • Description
  • COA
  • User Guide
  • Formula
  • Parameters
Catalog Number B1278-033

With

L-Glutamine,High Glucose,Phenol Red ,

Sodium Pyruvate

Without HEPES
Appearance Clear reddish solution
Product Size 500ML
Unit-Price($) 11.8
Triple 0.1µm sterile-filtered
Storage conditions 2°C to 8°C (protect from light)
Shipping conditions Room Temperature
Shelf life 12 months from date of manufacture

Compared to other basal media, F-10 contains a wider variety of components, including zinc, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-10 has led to a variety of improved formulations, such as Ham's F-12 Nutrient Mixture (F-12). F-10 contains no proteins or growth factors. Therefore, F-10 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-10 uses a sodium bicarbonate buffer system (1.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

CAUTION

Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.





Ham's F-10 Nutrient Mix    B1278-033

Components

Molecular Weight

Concentration (mg/L)

mM

Amino   Acids

Glycine

75.0

7.5

0.1

L-Alanine

89.0

9.0

0.101123594

L-Arginine   hydrochloride

211.0

211.0

1.0

L-Asparagine-H2O

150.0

15.0

0.1

L-Aspartic   acid

133.0

13.0

0.09774436

L-Cysteine

121.0

25.0

0.20661157

L-Glutamic   Acid

147.0

14.7

0.1

L-Glutamine

146.0

146.0

1

L-Histidine   hydrochloride-H2O

210.0

23.0

0.10952381

L-Isoleucine

131.0

2.6

0.019847328

L-Leucine

131.0

13.0

0.099236645

L-Lysine   hydrochloride

183.0

29.0

0.15846995

L-Methionine

149.0

4.5

0.030201342

L-Phenylalanine

165.0

5.0

0.030303031

L-Proline

115.0

11.5

0.1

L-Serine

105.0

10.5

0.1

L-Threonine

119.0

3.6

0.0302521

L-Tryptophan

204.0

0.6

0.0029411765

L-Tyrosine   disodium salt dihydrate

261.0

2.62

0.010038313

L-Valine

117.0

3.5

0.02991453

Vitamins

Biotin

244.0

0.024

9.836066E-5

Choline   chloride

140.0

0.7

0.005

D-Calcium   pantothenate

477.0

0.7

0.0014675052

Folic   Acid

441.0

1.3

0.0029478457

Niacinamide

122.0

0.6

0.004918033

Pyridoxine   hydrochloride

206.0

0.2

9.708738E-4

Riboflavin

376.0

0.4

0.0010638298

Thiamine   hydrochloride

337.0

1.0

0.002967359

Vitamin   B12

1355.0

1.4

0.0010332103

i-Inositol

180.0

0.5

0.002777778

Inorganic   Salts

Calcium   Chloride (CaCl2) (anhyd.)

111.0

33.3

0.29999998

Cupric   sulfate (CuSO4-5H2O)

250.0

0.0025

1.0E-5

Ferric   sulfate (FeSO4-7H2O)

278.0

0.834

0.003

Magnesium   Sulfate (MgSO4) (anhyd.)

120.0

74.62

0.6218334

Potassium   Chloride (KCl)

75.0

285.0

3.8

Potassium   Phosphate monobasic (KH2PO4)

136.0

83.0

0.6102941

Sodium   Bicarbonate (NaHCO3)

84.0

1200.0

14.285714

Sodium   Chloride (NaCl)

58.0

7400.0

127.586205

Sodium   Phosphate dibasic (Na2HPO4) anhydrous

142.0

153.7

1.0823944

Zinc   sulfate (ZnSO4-7H2O)

288.0

0.03

1.0416666E-4

Other   Components

D-Glucose   (Dextrose)

180.0

1100.0

6.111111

Hypoxanthine   Na

159.0

4.7

0.029559746

Lipoic   Acid

206.0

0.2

9.708738E-4

Phenol   Red

376.4

1.2

0.0031880978

Sodium   Pyruvate

110.0

110.0

1.0

Thymidine

242.0

0.7

0.002892562


Price: $11.80
Catalog Number: B1278-033
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