Ham's F-10 Nutrient Mix
- Detail
- Description
- COA
- User Guide
- Formula
- Parameters
| Catalog Number | B1278-033 |
|
With |
L-Glutamine,High Glucose,Phenol Red , Sodium Pyruvate |
| Without | HEPES |
| Appearance | Clear reddish solution |
| Product Size | 500ML |
| Unit-Price($） | 11.8 |
| Triple 0.1µm sterile-filtered | |
| Storage conditions | 2°C to 8°C (protect from light) |
| Shipping conditions | Room Temperature |
| Shelf life | 12 months from date of manufacture |
Compared to other basal media, F-10 contains a wider variety of components, including zinc, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-10 has led to a variety of improved formulations, such as Ham's F-12 Nutrient Mixture (F-12). F-10 contains no proteins or growth factors. Therefore, F-10 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-10 uses a sodium bicarbonate buffer system (1.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
CAUTION
Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
|
Ham's F-10 Nutrient Mix B1278-033 |
|||
|
Components |
Molecular Weight |
Concentration (mg/L) |
mM |
|
Amino Acids |
|||
|
Glycine |
75.0 |
7.5 |
0.1 |
|
L-Alanine |
89.0 |
9.0 |
0.101123594 |
|
L-Arginine hydrochloride |
211.0 |
211.0 |
1.0 |
|
L-Asparagine-H2O |
150.0 |
15.0 |
0.1 |
|
L-Aspartic acid |
133.0 |
13.0 |
0.09774436 |
|
L-Cysteine |
121.0 |
25.0 |
0.20661157 |
|
L-Glutamic Acid |
147.0 |
14.7 |
0.1 |
|
L-Glutamine |
146.0 |
146.0 |
1 |
|
L-Histidine hydrochloride-H2O |
210.0 |
23.0 |
0.10952381 |
|
L-Isoleucine |
131.0 |
2.6 |
0.019847328 |
|
L-Leucine |
131.0 |
13.0 |
0.099236645 |
|
L-Lysine hydrochloride |
183.0 |
29.0 |
0.15846995 |
|
L-Methionine |
149.0 |
4.5 |
0.030201342 |
|
L-Phenylalanine |
165.0 |
5.0 |
0.030303031 |
|
L-Proline |
115.0 |
11.5 |
0.1 |
|
L-Serine |
105.0 |
10.5 |
0.1 |
|
L-Threonine |
119.0 |
3.6 |
0.0302521 |
|
L-Tryptophan |
204.0 |
0.6 |
0.0029411765 |
|
L-Tyrosine disodium salt dihydrate |
261.0 |
2.62 |
0.010038313 |
|
L-Valine |
117.0 |
3.5 |
0.02991453 |
|
Vitamins |
|||
|
Biotin |
244.0 |
0.024 |
9.836066E-5 |
|
Choline chloride |
140.0 |
0.7 |
0.005 |
|
D-Calcium pantothenate |
477.0 |
0.7 |
0.0014675052 |
|
Folic Acid |
441.0 |
1.3 |
0.0029478457 |
|
Niacinamide |
122.0 |
0.6 |
0.004918033 |
|
Pyridoxine hydrochloride |
206.0 |
0.2 |
9.708738E-4 |
|
Riboflavin |
376.0 |
0.4 |
0.0010638298 |
|
Thiamine hydrochloride |
337.0 |
1.0 |
0.002967359 |
|
Vitamin B12 |
1355.0 |
1.4 |
0.0010332103 |
|
i-Inositol |
180.0 |
0.5 |
0.002777778 |
|
Inorganic Salts |
|||
|
Calcium Chloride (CaCl2) (anhyd.) |
111.0 |
33.3 |
0.29999998 |
|
Cupric sulfate (CuSO4-5H2O) |
250.0 |
0.0025 |
1.0E-5 |
|
Ferric sulfate (FeSO4-7H2O) |
278.0 |
0.834 |
0.003 |
|
Magnesium Sulfate (MgSO4) (anhyd.) |
120.0 |
74.62 |
0.6218334 |
|
Potassium Chloride (KCl) |
75.0 |
285.0 |
3.8 |
|
Potassium Phosphate monobasic (KH2PO4) |
136.0 |
83.0 |
0.6102941 |
|
Sodium Bicarbonate (NaHCO3) |
84.0 |
1200.0 |
14.285714 |
|
Sodium Chloride (NaCl) |
58.0 |
7400.0 |
127.586205 |
|
Sodium Phosphate dibasic (Na2HPO4) anhydrous |
142.0 |
153.7 |
1.0823944 |
|
Zinc sulfate (ZnSO4-7H2O) |
288.0 |
0.03 |
1.0416666E-4 |
|
Other Components |
|||
|
D-Glucose (Dextrose) |
180.0 |
1100.0 |
6.111111 |
|
Hypoxanthine Na |
159.0 |
4.7 |
0.029559746 |
|
Lipoic Acid |
206.0 |
0.2 |
9.708738E-4 |
|
Phenol Red |
376.4 |
1.2 |
0.0031880978 |
|
Sodium Pyruvate |
110.0 |
110.0 |
1.0 |
|
Thymidine |
242.0 |
0.7 |
0.002892562 |