BIOEXPLORER®
Ham's F-12 Nutrient Mix
  • Detail
  • Description
  • COA
  • User Guide
  • Formula
  • Parameters
Catalog Number B1299-109

With

L-Glutamine ,Sodium Pyruvate ,Phenol Red
Without HEPES
Appearance Clear reddish solution
PH   
7.7
Product Size 500ML
Unit-Price($) 11.8
Triple 0.1µm sterile-filtered
Storage conditions 2°C to 8°C (protect from light)
Shipping conditions Room Temperature
Shelf life 12 months from date of manufacture

Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell planting of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. BIOEXPLORER® Life Scienses   offers a variety of   F-12 modifications for a range of cell culture applications.

Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-12 has led to a variety of improved formulations.

Product Intended UseFor in vitro diagnostic use.

CAUTION

Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.


Ham's F-12 Nutrient Mix--- B1299-109

Components

Molecular Weight

Concentration (mg/L)

mM

Amino Acids

Glycine

75.0

7.5

0.1

L-Alanine

89.0

8.9

0.099999994

L-Arginine hydrochloride

211.0

211.0

1.0

L-Asparagine-H2O

150.0

15.0

0.1

L-Aspartic acid

133.0

13.3

0.1

L-Cysteine hydrochloride-H2O

176.0

35.12

0.19954544

L-Glutamic Acid

147.0

14.7

0.1

L-Glutamine

146.0

146.0

1.0

L-Histidine hydrochloride-H2O

210.0

21.0

0.1

L-Isoleucine

131.0

4.0

0.030534351

L-Leucine

131.0

13.1

0.1

L-Lysine hydrochloride

183.0

36.5

0.19945355

L-Methionine

149.0

4.5

0.030201342

L-Phenylalanine

165.0

5.0

0.030303031

L-Proline

115.0

34.5

0.3

L-Serine

105.0

10.5

0.1

L-Threonine

119.0

11.9

0.099999994

L-Tryptophan

204.0

2.04

0.01

L-Tyrosine

181.0

5.4

0.029834256

L-Valine

117.0

11.7

0.1

Vitamins

Biotin

244.0

0.0073

2.9918034E-5

Choline chloride

140.0

14.0

0.1

D-Calcium pantothenate

477.0

0.5

0.001048218

Folic Acid

441.0

1.3

0.0029478457

Niacinamide

122.0

0.036

2.9508196E-4

Pyridoxine hydrochloride

206.0

0.06

2.9126214E-4

Riboflavin

376.0

0.037

9.8404256E-5

Thiamine hydrochloride

337.0

0.3

8.9020777E-4

Vitamin B12

1355.0

1.4

0.0010332103

i-Inositol

180.0

18.0

0.1

Inorganic Salts

Calcium Chloride (CaCl2-2H2O)

147.0

44.0

0.2993197

Cupric sulfate (CuSO4-5H2O)

250.0

0.0025

1.0E-5

Ferric sulfate (FeSO4-7H2O)

278.0

0.834

0.003

Magnesium Chloride (MgCl2-6H2O)

203.0

122.0

0.6009852

Potassium Chloride (KCl)

75.0

223.6

2.9813335

Sodium Bicarbonate (NaHCO3)

84.0

1176.0

14.0

Sodium Chloride (NaCl)

58.0

7599.0

131.01724

Sodium Phosphate dibasic (Na2HPO4) anhydrous

142.0

142.0

1.0

Zinc sulfate (ZnSO4-7H2O)

288.0

0.863

0.0029965276

Other Components

D-Glucose (Dextrose)

180.0

1802.0

10.011111

Hypoxanthine

136.0

4.0

0.029411765

Linoleic Acid

280.0

0.084

2.9999999E-4

Lipoic Acid

206.0

0.21

0.0010194174

Phenol Red

376.4

1.2

0.0031880978

Putrescine 2HCl

161.0

0.161

0.001

Sodium Pyruvate

110.0

110.0

1.0

Thymidine

242.0

0.7

0.002892562

Price: $11.80
Catalog Number: B1299-109
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