MEM Medium
- Detail
- Description
- COA
- User Guide
- Formula
- Parameters
| Catalog Number | B1126-046 |
| With | L-glutamine, Phenol Red |
| Without | HEPES |
| Appearance | Clear reddish solution |
| Product Size | 500ML |
| PH | 7.4 |
| Unit-Price($） | 11.3 |
| Triple 0.1µm sterile-filtered | |
| Storage conditions | 2°C to 8°C (protect from light) |
| Shipping conditions | Room Temperature |
| Shelf life | 12 months from date of manufacture |
MEM (Minimum Essential Medium) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. BIOEXPLORER®offer a variety of MEM modifications for a range of cell culture applications. MEM was developed by Harry Eagle, based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow's MEM, MEM α, DMEM, and Temin's Modification. MEM is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2. This product is made with Earle's salts. MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum(FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
CAUTION
Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
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MEM B1126-046 |
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Components |
Molecular Weight |
Concentration (mg/L) |
mM |
|
Amino Acids |
|||
|
L-Arginine hydrochloride |
211.0 |
126.0 |
0.5971564 |
|
L-Cystine 2HCl |
313.0 |
31.0 |
0.09904154 |
|
L-Glutamine |
146.0 |
292.0 |
2.0 |
|
L-Histidine hydrochloride-H2O |
210.0 |
42.0 |
0.2 |
|
L-Isoleucine |
131.0 |
52.0 |
0.39694658 |
|
L-Leucine |
131.0 |
52.0 |
0.39694658 |
|
L-Lysine hydrochloride |
183.0 |
73.0 |
0.3989071 |
|
L-Methionine |
149.0 |
15.0 |
0.10067114 |
|
L-Phenylalanine |
165.0 |
32.0 |
0.19393939 |
|
L-Threonine |
119.0 |
48.0 |
0.40336135 |
|
L-Tryptophan |
204.0 |
10.0 |
0.04901961 |
|
L-Tyrosine disodium salt dihydrate |
261.0 |
52.0 |
0.19923371 |
|
L-Valine |
117.0 |
46.0 |
0.3931624 |
|
Vitamins |
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|
Choline chloride |
140.0 |
1.0 |
0.007142857 |
|
D-Calcium pantothenate |
477.0 |
1.0 |
0.002096436 |
|
Folic Acid |
441.0 |
1.0 |
0.0022675737 |
|
Niacinamide |
122.0 |
1.0 |
0.008196721 |
|
Pyridoxal hydrochloride |
204.0 |
1.0 |
0.004901961 |
|
Riboflavin |
376.0 |
0.1 |
2.6595744E-4 |
|
Thiamine hydrochloride |
337.0 |
1.0 |
0.002967359 |
|
i-Inositol |
180.0 |
2.0 |
0.011111111 |
|
Inorganic Salts |
|||
|
Calcium Chloride (CaCl2) (anhyd.) |
111.0 |
200.0 |
1.8018018 |
|
Magnesium Sulfate (MgSO4) (anhyd.) |
120.0 |
97.67 |
0.8139166 |
|
Potassium Chloride (KCl) |
75.0 |
400.0 |
5.3333335 |
|
Sodium Bicarbonate (NaHCO3) |
84.0 |
2200.0 |
26.190475 |
|
Sodium Chloride (NaCl) |
58.0 |
6800.0 |
117.24138 |
|
Sodium Phosphate monobasic (NaH2PO4-H2O) |
138.0 |
140.0 |
1.0144928 |
|
Other Components |
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|
D-Glucose (Dextrose) |
180.0 |
1000.0 |
5.5555553 |
|
Phenol Red |
376.4 |
10.0 |
0.026567481 |