BIOEXPLORER®
MEM Medium
  • Detail
  • Description
  • COA
  • User Guide
  • Formula
  • Parameters
Catalog Number B1126-046
With L-glutamine, Phenol Red
Without HEPES
Appearance Clear reddish solution
Product Size 500ML
PH 7.4
Unit-Price($) 11.3
Triple 0.1µm sterile-filtered
Storage conditions 2°C to 8°C (protect from light)
Shipping conditions Room Temperature
Shelf life 12 months from date of manufacture

MEM (Minimum Essential Medium) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. BIOEXPLORER®offer a variety of MEM modifications for a range of cell culture applications. MEM was developed by Harry Eagle, based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow's MEM, MEM α, DMEM, and Temin's Modification. MEM is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2. This product is made with Earle's salts. MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum(FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

CAUTION

Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.





MEM   B1126-046

Components

Molecular Weight

Concentration (mg/L)

mM

Amino   Acids

L-Arginine   hydrochloride

211.0

126.0

0.5971564

L-Cystine   2HCl

313.0

31.0

0.09904154

L-Glutamine

146.0

292.0

2.0

L-Histidine   hydrochloride-H2O

210.0

42.0

0.2

L-Isoleucine

131.0

52.0

0.39694658

L-Leucine

131.0

52.0

0.39694658

L-Lysine   hydrochloride

183.0

73.0

0.3989071

L-Methionine

149.0

15.0

0.10067114

L-Phenylalanine

165.0

32.0

0.19393939

L-Threonine

119.0

48.0

0.40336135

L-Tryptophan

204.0

10.0

0.04901961

L-Tyrosine   disodium salt dihydrate

261.0

52.0

0.19923371

L-Valine

117.0

46.0

0.3931624

Vitamins

Choline   chloride

140.0

1.0

0.007142857

D-Calcium   pantothenate

477.0

1.0

0.002096436

Folic   Acid

441.0

1.0

0.0022675737

Niacinamide

122.0

1.0

0.008196721

Pyridoxal   hydrochloride

204.0

1.0

0.004901961

Riboflavin

376.0

0.1

2.6595744E-4

Thiamine   hydrochloride

337.0

1.0

0.002967359

i-Inositol

180.0

2.0

0.011111111

Inorganic   Salts

Calcium   Chloride (CaCl2) (anhyd.)

111.0

200.0

1.8018018

Magnesium   Sulfate (MgSO4) (anhyd.)

120.0

97.67

0.8139166

Potassium   Chloride (KCl)

75.0

400.0

5.3333335

Sodium   Bicarbonate (NaHCO3)

84.0

2200.0

26.190475

Sodium   Chloride (NaCl)

58.0

6800.0

117.24138

Sodium   Phosphate monobasic (NaH2PO4-H2O)

138.0

140.0

1.0144928

Other   Components

D-Glucose   (Dextrose)

180.0

1000.0

5.5555553

Phenol   Red

376.4

10.0

0.026567481

Price: $11.30
Catalog Number: B1126-046
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